Regulation of expression of Escherichia coli pilus K99

Confirmed nucleotide sequence of fanF of Escherichia coli K99 fimbriae.

Analysis of a naturally occurring K99+ enterotoxigenic Escherichia coli strain that fails to produce K99.

A spontaneously occurring field isolate of enterotoxigenic Escherichia coli that was genotypically K99+ but phenotypically K99- was analyzed for the reason that it did not express K99. The defect, which was cis active, was located within an area 5' to the first gene required for K99 biogenesis and was the result of the deletion of a single base pair.

Effect of sodium acetate on expression of K99 pili by Escherichia coli.

Sodium acetate suppressed K99 production in Escherichia coli strains cultured on a minimal medium, as determined by seroagglutination and enzyme-linked immunosorbent assay. The greatest suppression occurred when the medium contained both sodium acetate and glucose. Glucose alone did not suppress K99 production.

Characterization of the Escherichia coli AF/R1 pilus operon: novel genes necessary for transcriptional regulation and for pilus-mediated adherence.

We isolated the genetic determinant of AF/R1 pilus production in attaching/effacing Escherichia coli RDEC-1 and identified seven genes required for pilus expression and function. DNA sequence analysis of the structural subunit gene afrA corrected an error in the published sequence and extended homology with the F18 pilus subunit of pig edema E. coli strains. AfrB and AfrC, encoded downstream fr...

Structure of the Cs1 Pilus of Enterotoxigenic Escherichia Coli

1 STRUCTURE OF THE CS1 PILUS OF ENTEROTOXIGENIC ESCHERICHIA COLI 1 REVEALS STRUCTURAL POLYMORPHISM 2 3 Running title: Structure of the ETEC CS1 pilus 4 5 Vitold E. Galkin*, Subramaniapillai Kolappan, Dixon Ng, ZuSheng Zong, Juliana Li, 6 Xiong Yu, Edward H. Egelman and Lisa Craig* 7 8 Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, 9 Charlottesville, Vi...